& >Basic information
Song Dynasty, Chinese name: trihydroxymethylaminomethane
<, English name: r
<, English alias: b,rr
<, C number:
< , Molecular formula: C
< , Linear molecular formula: C(C)
< , Molecular weight: .
< , Purity: &.%
< , Grade: BR
< , No.: C
< , No. B:
, No. C:
, .>Quality specifications
< , Purity:&.%
< , Insoluble matter: qualified
< , & .>Preparation method< c= < , first dissolve r with a small amount of double distilled water (~), add C, then use C or adjust to., and finally double distill Add water to. This solution is a stock solution and should be stored in a refrigerator at ℃.
<, Note: The value of rc changes with temperature, so you should wait until room temperature before measuring, so that the measured results are more reliable.
< c= .>Uses
< , r buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses. r was used for protein crystal growth under different conditions. The low ionic strength characteristic of r buffer can be used for the formation of intermediate fibers of lamin in C. elegans (C). r is also one of the main components of protein electrophoresis buffer. In addition, r is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. r is also used as a titration standard.
< c=r < c= Buffering Characteristics
< , r is a weak base, at room temperature (℃), its is. According to buffer theory, the effective buffering range of r buffer is from. to. between.
< , the aqueous solution of r base is around . Generally, hydrochloric acid is added to adjust the value to the required value, and a buffer of this value can be obtained. But at the same time, attention should be paid to the effect of temperature on r.
< c= & < c=r < c= &
Trishydroxymethylaminomethane